Ovulation is the release of an egg from the ovary. The egg then passes into the fallopian tube where it is ready to be fertilized. A baby is conceived when the male sperm successfully fertilizes the female egg. When a woman is about to ovulate, her body releases a large amount of a hormone called L.H. (Luteinising Hormone). L.H. is always present in your urine but the levels increase (surge) in the middle of your cycle, causing you to release an egg from the ovary.
The One Step Ovulation Test is an immunochromatographic in-vitro assay for the qualitative and semi-quantitative determination of the human luteinizing hormone (LH) in urine to predict the time of ovulation in women. The test detects the sharp increase in LH concentration in urine, the so called “LH surge” which precedes ovulation. Conception is most likely to occur within 36 hours following the LH surge.
The first step is to determine the length of your menstrual cycle. This is the number of days from the first day of your menstrual bleeding to the day before your next bleeding starts. Please refer to the chart to determine when you should start testing. If your cycle is shorter than 21 days or longer than 38 days, consult your doctor. If you do not know your cycle length, you may begin testing 11 days after your first period since the average cycle length is 28 days. Perform 1 test each day over a 5 day period, or until the LH surge has been detected.
1. Do not use first morning urine samples as LH is synthesized in your body early in the morning. It will not show up in your urine until later in the day.
2. The best time to collect your urine is between 10am and 8pm.
3. Collect urine at about the same time each day.
4. Reduce liquid intake about 2 hours before collecting your urine as a diluted urine sample can prevent the test from detecting the LH surge.
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