CK-MB test is a sandwich immunoassay. When serum sample is added to sample pad, it moves through the conjugate pad and mobilizes gold anti-CK-MB conjugate that is coated on the conjugate pad. The mixture moves along the membrane by capillary action and reacts with the anti-CK_x005f MB antibody that is coated on the test region. If CK-MB is present at levels of 7.0 ng/ml or greater, the result is the formation of a colored band in the test region. If there is no CK-MB in the sample, the area will remain colorless. The sample continues to move to the control area and forms a pink to purple color, indicating the test is working and the result is valid.
You may need this test if your healthcare provider thinks you are having a heart attack. Symptoms of a heart attack often include:
Pain or discomfort in the chest, such as a squeezing sensation or feeling of fullness
Pain in the neck, back, left arm, or jaw
Shortness of breath
Lightheadedness or dizziness
Nausea or vomiting
Sudden sweating
Tiredness
Your healthcare provider may also order a test to measure cardiac troponin (CTn). This test is more often used than CK-MB because it more specifically shows heart damage.
Your healthcare provider may also order an electrocardiogram (ECG). This is done to measure electrical activity in your heart and help diagnose a heart attack.